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CABRI:ICLC |
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Accession_number |
ICLC HYL95001
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Cell_line_name |
AF-2 clone 9B5
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Brief_description |
Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: myeloma
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Description |
Species: mouse, BALB/c; Tissue: hybrid, B cells x myeloma; Tumor: myeloma
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Depositor |
Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy.
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Bibliographic_reference |
-
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Morphology |
lymphoblast, grown in suspension
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 3 and 9 x 105 cells/ml; split 1:5-1:10; resuscitate with medium containing 20% FCS, with a feeder layer of 3T3. 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
Hybridoma producing IgM monoclonal antibody specific for AF-2 protein.
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Release_conditions |
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by isoenzymes: confirmed as mouse with G6PD, NP, MD
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Exp Cell Res 1991;193:411-419 [PMID: 2004654
]
J Cell Physiol 1991;149:567-574 [PMID: 1744180
]
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